Free western blot quantification software licor biosciences. Moving the odyssey clx imager always be sure to disconnect the power to the instrument before moving it as described in disconnecting the power in chapter 2. Odyssey clx manuals, application notes, software, and webinars. Odyssey clx infrared imaging system from licor biosciences. The linear range spanned only 5 dilutions, a 32fold range orange. Thus, to get an image like that shown in figure 8 top of next page that excludes the empty space from the blot, one would need to shrink the viewing window within the odyssey software before using the export image view function. Try empiria studio software today and enhance your odyssey workflow with the only western blot analysis software.
Image acquisition and file organization software for use with licor imaging systems. To achieve this adaptation, the hrpconjugated streptavidin provided in the kit is simply replaced with irdye 800cw streptavidin, and the arrays are. The odyssey clx system requires an area approximately 54 cm w 21. Image studio lite software from licor biosciences biocompare. Founded to sell, implement and support dynamics gp in the pittsburgh region, we have grown to having almost 200 customers across the united states.
The captured the digital data results provide over six logs of linear range. Licor image studio software for accurate data quantification. Licor ir based system is obviously elegant system and more quantitative. Total protein analysis as a reliable loading control for.
Only the first 23 dilutions orange fell with the linear range 48 fold range, r 2 0. Select bands quickly and quantify signal intensity without altering raw data. If you have questions about how our administrative software solutions would work for your agency, stop by our booth for more information. Image studio lite is a free software package from licor biosciences aimed at life scientists that want to analyze gels, western blots, dot blots, and other similar lab outputs. Licorodysseysignup web calendar brown bear software. I am still getting familiar with the software and quantitative analysis. Try free image studio lite software for your western blot quantification.
Licor odyssey clx the new odyssey clx infra red imaging system from licor biosciences improves your quantitative analysis for detecting proteins on membranes, in cells, in tissue, invivo etc. Computer software cables cords manual chemiluminescence and. Get accurate results from more than 6 logs of linear dynamic range for both nir fluorescent and ecl western blots. Used licor odyssey sa infrared imaging system 3014309.
We use cookies on the website to provide you with the most relevant information. Read this entire manual before using the odyssey fc imager. Odyssey imagers, pearl imagers, cdigit blot scanner, and aerius imager. Incell western protocol incell western assays icw also known as incell elisa ice allow researchers a simple and rapid assay method for detection of biomarkers and signaling proteins in whole cells. Licor odyssey western blots hi everyone, i have western blot pictures, either as a two channel picture or two separate pictures of the two channels 700800nm. We also use it for emsa, cell migration, coomassie stained gel pictures etc. Get clearer answers with accurate, replicable western blots and many other assays.
Although the lite version is free, there is a more comprehensive paid version of the software that aims to easily integrate with the apparatus that licor also sells. Image studio lite is used for basic western blot analysis, but does not operate your licor instrument. Licor, odyssey, mpx, incell western, and irdye trademarks contained in the software product are. Licor biosciences, odyssey infrared imaging system b446. More details about the software image studio lite 5. Licor biosciences li6200 portable photosynthesis system. What is the best detection system for western blots odyssey clx but cant fit it in the budget. The major advantage with this solution is accurate quantification over a broad dynamic range, up to 6 orders with high sensitivity. Li6400xt training and resources licor environmental.
Multiuser licenses are available so that you can use image studio on multiple computers. Licor biosciences pioneering family of irdye infrared dyes are synthesized. What is the best detection system for western blots free software package from licor biosciences aimed at life scientists that want to analyze gels, western blots, dot blots, and other similar lab outputs. With the hood fully open, the odyssey clx imager requires 74 cm 29 of vertical clearance. Perform small animal imaging with nearinfrared fluorescence and bioluminescence detection. The detection quality is excellent and the software is both easy to use and provides clear quantification. Export image view as found from the file menu figure 5. Does anybody have a lot of experience with image studio lite by li. Image studio software is compatible with most licor imaging systems, including the odyssey imaging systems, pearl imaging system, and cdigit blot. We show the full front view of the licor odyssey 9120 infrared imaging system, looking. The consistent digital images produced by the imaging system cut out the hassle of film, while providing clear near infrared results. If you experience connection problems, wait several minutes and then click connect again. Odyssey clx infrared imaging system operators manual.
This is a quick look at the free densitometry software from licor. It gives a basic overview of the functionality and i run through the analysis of a gel. Designed specifically for western blot quantification, image studio lite is simple, intuitive software. Researchers have already downloaded this software over 80000 times. Blots and gels were imaged using an odyssey infrared imaging system scan resolution. The only negative is that the handling of the software could be based more on windows. Odyssey software was founded in 1996 by mark gentile and originally focused on building software development tools.
Used licor odyssey 9120 infrared imaging system 9120 lab. This product model number 2800 is a cemarked product. Infrared detection gives you the quantitative analysis and wide linear dynamic range that chemiluminescence and visible fluorescence cannot. Odyssey software will be exhibiting at the open minds tii conference in philadelphia, pa on october 28th and 29th. Automated western blot infrared imaging system model 9260.
Image studio lite is western blot analysis software that is compatible with most image files and systems and is available for free download from the licor. Mar 04, 2014 this video tutorial demonstrates the acquire ribbon, which contains all of the controls for acquiring new images with image studio 4. I want to analyze them, doing a density intensity plot over a sample lane. Odyssey software mobile device management wikipedia. Licor lab instruments, equipment, and accessories biosurplus.
Used for visualizing images of genes, gene fragments, and gene products. With odyssey clx infrared imaging system, you can get clearer answers with accurate reproducible western blots and more. Fill out the form below to receive an email with a link to download your free image studio lite software. Licor image studio lite free densitometry software youtube. Odyssey clx application protocols manual vanderbilt university. The only western blot analysis software that guides you from validation through statistical analysis for reliable results. This video tutorial demonstrates the acquire ribbon, which contains all of the controls for acquiring new images with image studio 4. The licor odyssey 9120 delivers accurate and reproducible results with western blot and more. Odyssey clx application and technical notes show the versatility of the clx.
These are products by a single manufacturer or a list of manufacturers. Odyssey applications featuring irdye infrared dyes infrared dyes are a key performance advantage of the odyssey system. Licor odyssey clx infrared imaging system model 9140. Mar 18, 2015 this is a quick look at the free densitometry software from licor. Software upgrades are free stay current for the latest innovations. Since the odyssey is a two channel instrument it can record data for either wavelength separately or both wavelengths simultaneously. When initial screen has loaded, click on file and select new from the dropdown menu.
The captured the digital data results provide over six logs of linear. Originally developed for chemiluminescent detection, many proteome profiler arrays are amenable to nearinfrared nir fluorescence detection using the licor odyssey infrared imaging system. Take back your benchtop and capture quality ecl data. If you are using licor odyssey machine to scan your western blot membrane it may be. Licor biosciences pioneering family of irdye infrared dyes are synthesized with reactive functional groups that enable easy covalent coupling to antibodies and other biomolecules. Download image studio lite software licor biosciences. A a free training dvd and manuals both hardcopy and digital format are included with the purchase of the li6400xt, as well as numerous online resources such as webinars and application notes, which.
Simple, free western blot signal quantification software. The li6800 includes easytolearn software that makes it easier to understand the complexities of photosynthesis measurements. Can be used to distinguish between normal and cancer cells. Licorir based system is obviously elegant system and more quantitative. Odyssey has sells, implements and supports cloudbased systems for human capital management, budgeting and planning and financial management. Download free image studio lite for western blot quantification. Its streamlined, ribbonbased design empowers you to quickly analyze and export images. Manuals, software information, and webinars are also listed.
For conformity information, contact licor support at biosupport. However, it now focuses on developing software products that enable developers. The only negative is that the handling of the software could be based more on windowshandling e. It has two lasers, which operate at 680 and 780nm, for the detection of infrared fluorescent dyes. The software searches for and discovers the odyssey clx imager on the same lan automatically.
Odyssey clx imaging system odyssey fc imaging system pearl trilogy small animal imager cdigit. A a free training dvd and manuals both hardcopy and digital format are included with the purchase of the li6400xt, as well as numerous online resources such as webinars and application notes, which are continuously updated. Use of proteome profiler arrays with licor detection. When initial screen has loaded, click on file and select new from. If you experience connection problems, wait several minutes and then. This licor biosciences li6200 portable photosynthesis system is used and in excellent condition. Infrared detection gives you the quantitative analysis and wide linear dynamic. The odyssey fc imager is intended to be used by qualified personnel. Incell western protocol incell western assays icw also known as incell elisa ice allow researchers a simple and rapid assay method for detection of biomarkers and signaling proteins in whole cells using antibodies.
This protocol is designed for the odyssey infrared imaging system by licor. This unit is missing a handle knob, and the ctrl and shift buttons do not operate. Odyssey has a new hard drive installed for optimal life and performance. This leads to better data that you can be confident in. The same intuitive acquisition software is compatible with these licor imaging systems. Used other licor odyssey imager for sale dotmed listing. Licor licor odyssey 9120 infrared imaging system imager. The next time you connect, the software will automatically detect the odyssey clx imager. Quantitative analysis of the spots is fast and easy. We are unable to display the form due to your browsers privacy settings. The licor odyssey clx imaging system model 9140 is designed to be flexible and consistent while performing different experiments.
Image studio is compatible with both mac and pc computers. Licor biosciences is an international biotech company which designs, manufactures, and markets instruments, measurement systems, and software for biological and environmental research, and develops relevant measurement methodologies and techniques. All other dilutions were affected by saturation purple. Image studio lite is western blot analysis software that is compatible with most image files and systems and is available for free download from the licor website. In the prompt box that opens, select browse from the right side.